The best Side of attune flow cytometer
The best Side of attune flow cytometer
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Facts-enriched characterization of mobile populations. Morphological info from illustrations or photos can include into the richness of flow cytometry information. By way of example, the figure exhibits an otherwise standard apoptosis assay working with Annexin V and PI, including cell imaging to characterize cells in Each and every population to reveal morphologically distinct attributes. These insights couldn't are obtained from multiplex staining alone.
As samples are acquired within the Attune CytPix Flow Cytometer, the significant-speed brightfield digital camera captures and merchants visuals of detected gatherings in a charge as much as 6,000 images for every next, with regards to the flow rate and graphic sizing. For higher overall flexibility, Attune Cytometric Application allows you to modify the impression seize frequency as desired and select a particular gate for graphic seize, if needed.
Mix acoustic concentrating technologies with higher-parameter analysis for versatile spectral unmixing and conventional workflows.
The award-winning Invitrogen Attune CytPix Flow Cytometer brings together acoustic focusing which has a superior-speed brightfield digital camera for simultaneous large throughput flow cytometry and superior resolution brightfield imaging.
"An image isn't really very really worth a thousand dots, nonetheless it definitely really helps to see photographs. It is great to discover photographs that correlate with staining. For instance, AnnexinV-positive cells which have been morphologically various than healthy cells, or CD14+ monocytes which are more substantial and much more textured than CD3+/CD4+/CD14- lymphocytes.
Below, a skilled consumer has gated singlets confidently. Right after assessing the guide singlet gate, the CytPix graphic-derived parameter ParticleCount reveals this gate contains in excess of 4% aggregates.
Pair your Attune flow cytometer using an autosampler for amplified workflow effectiveness, and insert robotic integration for optimum throughput.
Our experts will layout panels for you as often times you would like—cost-free, no item obtain needed.
The Attune NxT cytometer may be the dependable workhorse instrument your lab can count on for reliable and trusted general performance. The Attune CytPix product incorporates a superior-velocity brightfield camera and AI-pushed graphic Investigation program, with modern upgrades such as machine learning resources and an intuitive user interface, enabling enhanced data precision, accuracy, and more rapidly insights into read more sample biology.
Maybe most significantly, these occasions comprise cells of Obviously diverse phenotypes which may lead to incorrect conclusions with regards to double favourable activities (specifically in uncommon populations).
AI-pushed automatic picture Examination With all the Attune Cytometric Software translates celebration capabilities into distinct morphology parameters that could be combined with typical fluorescence and scatter parameters to expose very reproducible population adjustments and special subsets.
Application Take note: Automated impression Assessment cuts down person-to-person variability in flow cytometry gating approaches
Developed-in dimensionality reduction equipment improved resolution into populations. Fluorescently gated populations again-gated on to UMAP plot to confirm envisioned populace resolution in lysed whole human blood. User can watch situations from Every cluster for Visible confirmation of mobile phenotypes.
Versatile payment prepare from Agilent Economic Options let you have the important instrumentation you would like for rapid challenge, or to mature your lab.
At the heart of every Attune Flow Cytometer lies our groundbreaking acoustic focusing engineering, which utilizes seem waves to meticulously align cells throughout the flow cell. This ground breaking tactic surpasses regular hydrodynamic techniques, resulting in faster processing speeds, Outstanding info high-quality, and greater sample throughput.
As the cell line experienced Earlier been analyzed to the Attune CytPix Flow Cytometer, the researchers went again to the photographs and were able to doc the microbial an infection a minimum of 5 days earlier.
Agilent Worth Promise guarantees you no less than ten years of instrument use in the day you buy. Otherwise, We are going to credit score you Using the residual price of the system toward an upgraded model.
Here, we clearly show that by gating approaches making use of graphic-centered quantitative parameters can distinguish interacting cells from coincident situations to extra correctly review interacting cells.